Evaluation of Pseudoenantiomeric Mixed Carbonates as Efficient Fluorogenic Probes for Enantioselectivity Screening.

نویسندگان

  • Anna Zadlo
  • Dominik Koszelewski
  • Filip Borys
  • Ryszard Ostaszewski
چکیده

We report mixed carbonates as enzyme substrates and demonstrate their application as fluorogenic probes for lipase and esterase enantiopreference screening. By the application of pseudoenantiomers with distinct fluorescence behaviors, it is possible to evaluate the activity and enantiopreference of hydrolytic enzymes. In order to validate our method, enantioselectivities calculated from fluorometric measurements were compared with the results obtained from larger-scale kinetic resolution.

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عنوان ژورنال:
  • Chembiochem : a European journal of chemical biology

دوره 17 1  شماره 

صفحات  -

تاریخ انتشار 2016